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EMPTY VESSEL STERILIZATION-ESIP

To reduce the bioburden prior to charging a media it is imperative to sterilize the Fermenter / Bioreactor in empty condition, clean steam is directly introduced in vessel through spray balls, complete system along with all auxiliary lines/valves & sterile boundaries.

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INDEPENDENT STERILIZATION OF TRANSFER IN /ADDETIVE LINES

All the isolation valves & lines for transfer IN line is sterilized in ESIP & FSIP. When any additive is to be transferred post media sterilization, respective reservoir is attached to respective addition port & in situ SIP is done , to ensure the closed operation & aseptic transfer.

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CALIBRATION OF SENSORS

Before installing the sensors in Fermenter /Bioreactor, it has to be calibrated. Calibration of pH sensors for buffer 4 & 7, calibration of DO2 at 0% & 100% DO .

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MEDIA CHARGING

Media is charged In Fermenter transferred from media preparation area in non-sterile condition. And in Bioreactor it is charged from media preparation area through sterile filter.

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